MOLS7212 Proteomics Technologies and Applications
LECTURE, TUTORIAL and PRACTICAL TIMETABLE
Lectures: Thursdays 10:00 am - 12:00 pm July 29th - November 4th, either 9WW 102 or online
Tutorials/workshops: Fridays 10:00 am - 11:00 am July 30th - November 5th, either 3IR G240 or online
NOTE: the first scientific content lecture will be delivered in the first tutorial timeslot.
Practicals: Are held in a one-week block during semester break. You must be available for all of September 13th- 17th. Practical classes run about 6+ hours per day, between 9am – 5 pm. This is the equivalent of 3+ hours per week for the whole semester, we just do it all at once. Attendance is compulsory - if you are not able to attend the practical class all week, for any reason, please do not enrol in this unit.
The practical course includes differential display 2D gel electrophoresis, in-gel protein digestion, peptide spectrometry (MALDI-TOF/TOF and nanoESI-Linear ion trap), protein identification using Mascot and XTandem, shotgun proteomic analysis using SDS-PAGE protein fractionation, and label-free protein quantitation using normalized spectral abundance factors.
Up to date timetable information is found at timetables.mq.edu.au
All unit information is distributed using the unit website on ilearn, accessed via ilearn.mq.edu.au
MOLS7212 Proteomics Technologies and Applications
ASSESSMENT PROCESS 2021
Mini-Review Essay (Due 9am Friday September 3rd) - 15%
Topic for 2021: Compare and contrast the way in which proteomics studies are performed in current literature as opposed to those performed ten years ago.
- 1500 word mini-review article suitable for publication (not including abstract, keywords, references, diagrams, tables or figures, all of which are encouraged).
- Must conform to the Instructions for Authors for a review article submitted to “Journal of Proteomics”. Look up the Journal of Proteomics instructions and follow them. Make sure you read some review articles in the journal before you start writing, because that will give you a good template to work from.
Group Oral Tutorial Presentation – Various Dates 15%
- You will work in groups of two, so identify a partner as soon as possible.
- Choose one publication from the Tutorial Papers List (on a first-come first-served basis), which is found on a wiki on the iLearn site.
- The papers are to be presented on the date indicated, because they are sorted by subject material. There is a small number of general interest papers at the end of the list which can be presented on any date you wish to choose.
- A maximum of two group presentations will be scheduled for each week. If you put your name down on a particular date as the third group, you will miss out on presenting, so you will have wasted your time preparing a presentation.
- Present your critique of the topic as a short Powerpoint seminar. Aim for 10 min talking (12 minutes max), and there will be time for questions. We may adjust that schedule depending on class numbers.
- Summarise the paper. Presented in a short form so that other students in the class can understand what was done, and how, and why.
- Perform your own peer-review of your chosen paper - tell us whether you think this paper should have been published and why.
- Look up other relevant literature so you can discuss your chosen paper in context rather than in isolation.
- Participation in all other group’s topic presentations contributes to your final mark - if you turn up and ask questions, you will get a better mark. Since this constitutes a continuing assessment component, marks will not be finalised and released until the last presentation has been completed at the end of the semester.
- The research tutorial presentation can it be presented live, either in person or by zoom, and it will also be accented as a video presentation uploaded in advance to youtube. Pre-recorded videos must include figures, graphics, text (and some footage of the presenter). You can either record straight to video camera, or use software such as iMovie or Windows Movie Maker. Tutorial presentations will be recorded and made available via echo 360. It is best for both students to take part in presenting, and share the workload.
- An essential part of this task is organising yourselves so that everyone in the class has a time scheduled to present their work. A wiki will be set up in iLearn, and you will be able to put your name down. The papers are grouped by subject so that they follow the weekly lecture content fairly closely, to help reinforce your learning. Hence, a paper on a specific topic will be listed on a given date and can only be presented on that date.
- The exception to this is the general interest papers which can be presented on any date you wish to choose. If you choose to present one of the general interest papers, it is your job to copy and paste that information into the correct date on the wiki so that everyone knows what is going on.
- It is important to be proactive about this scheduling task, because the unit convenor will not be involved. If there are three or four group names down to present on one week, you need to talk among yourselves and fix the problem. Please be aware that we frequently have issues with students putting their name down and then withdrawing from the unit. That means timeslots go by unused and then there is not enough time for other students to present. Again, it is the responsibility of all students involved to make sure that we get two presentations each week.
Continuing assessment: Weekly Speaker Questions - 5%
- You will be given 5 minutes at the end of each lecture in which you are required to write down and submit a question concerning the lecture of the day. This must be relevant to the topic that has been presented.
Note that in the event of a Coronavirus global pandemic:
- You will be required to submit a written question after each lecture via iLearn, which must be relevant to the topic that has been presented. Each week there will be a link to a wiki under the Introduction and timetabling section of iLearn where you get to enter a question, next to your name. The wiki will be active from 12 midday when the lecture finishes until midnight of the next day, so you have plenty of time in which to listen to the recorded lecture (if necessary) and write down a sensible question. No late submissions will be accepted. Discussion of these will form our weekly revision session prior to the next new content lecture, so if there is something you don’t understand in a lecture, make sure you ask about it.
Mid-semester test – week 8 - 5%
- This will typically be a short quiz aimed at helping students assess their areas of strength and weakness prior to the final exam. It will be held after the midsemester break, date to be confirmed.
Practical Report (Due Monday October 4th) - 20%
- You must present your work in the format of a manuscript suitable for publication in Journal of Proteomics. This will be discussed in detail during the practical session.
Final Exam (2.5 hrs, date and time to be advised) - 40%
- 2.5hr exam covering all practical and theoretical components of MOLS7212
- Questions are a mix of long and short answer questions